What primary receptor cascades and in vitro signaling axes are modulated by Ipamorelin?

Pre-clinical studies indicate Ipamorelin engages primary pathways: Selectively activates pituitary and hypothalamic GHSR-1a receptors via G-protein coupled phospholipase C. Elevates intracellular inositol trisphosphate (IP3) and calcium mobilization in somatotroph cells. Additional investigations explore cellular migration, receptor binding affinities, and kinase activation in vitro.

What is the recommended reconstitution diluent and sterile handling protocol for Ipamorelin?

Lyophilized Ipamorelin cakes must be reconstituted using Bacteriostatic 0.9% Benzyl Alcohol USP. Introduce the diluent gently along the internal glass wall under a laminar flow hood, allowing passive dissolution without mechanical vortex shear or high-velocity liquid agitation.

Is Ipamorelin lawful to purchase and possess for laboratory research in the United Kingdom?

Yes. Non-scheduled synthetic research peptides like Ipamorelin are entirely legal to acquire, import, and hold for bona fide laboratory experimentation in the UK. However, under Regulation 46 of the Human Medicines Regulations 2012 (SI 2012/1916), supply or marketing for human clinical administration without MHRA authorization is strictly unlawful.

UK Academic MonographGrowth Hormone Secretagogues & GHRHIn Vitro Laboratory Only

Ipamorelin

Q: What is the CAS registry number, molecular formula, and mass of Ipamorelin?

Ipamorelin (Aib-His-D-2-Nal-D-Phe-Lys-NH2 Pentapeptide) is indexed in the CAS Registry under #170851-70-4. Its empirical molecular formula is C38H49N9O5 with a monoisotopic molecular weight of 711.86 g/mol. It is cataloged under the Growth Hormone Secretagogues & GHRH research classification.

Q: What are the storage temperature thresholds and solution kinetics for Ipamorelin?

Lyophilized vials require desiccated storage at -20°C desiccated (stable up to 24 months). Once reconstituted into an aqueous solvent, the peptide solution must be stored at 2°C to 8°C refrigerated; protect from light (stable 28 days) protected from UV degradation. The laboratory stability window is Enzymatically stable pentapeptide; keep tightly sealed in glass vials. Avoid repeated freeze-thaw cycles.

Aib-His-D-2-Nal-D-Phe-Lys-NH2 Pentapeptide

PubChem Molecular SpecsValidated

CAS Registry:170851-70-4

Chemical Formula:C38H49N9O5

Monoisotopic MW:711.86 g/mol

PubChem CID:9831659

Primary Amino Acid Sequence (5 residues)

N-terminus → C-terminus

1Aib

2His

3D-2-Nal

4D-Phe

5Lys-NH2

Pre-Clinical Overview

Ipamorelin is a synthetic pentapeptide and selective ghrelin receptor (GHSR-1a) agonist. Distinct from earlier GHRPs due to its selectivity: it stimulates somatotroph growth hormone release in vitro without triggering substantial elevation in cortisol, prolactin, or ACTH.

Independent Laboratory Audit

Verified UK Suppliers for Ipamorelin

5 Audited British & European Sources

Supplier	Assay Purity	Reagent Format	Dispatch Location	Notes
PharmaGrade Store	≥99.3% (HPLC)	vial	United Kingdom (Royal Mail 24)	Certified high-selectivity GHSR-1a reagent vials with third-party testing.
Direct Peptides	≥99.1% (HPLC)	vial	UK Hub & Europe	High-purity lyophilized vials and CJC/Ipamorelin laboratory research stacks.
Direct Sarms	≥98.8% (HPLC)	vial	United Kingdom Express	Analytical reference standard vials tested for GHSR-1a selectivity.
Peptide Works	≥99.0% (HPLC)	vial	Manchester UK Hub	Manchester synthesized pentapeptide vials accompanied by digital COA.
PharmaLab Global	≥99.5% (HPLC)	pen	London UK Hub	Triple-stage assayed vials and pre-calibrated multi-dose delivery pens.

Biochemical Mechanisms of Action

Molecular Signaling Pathways

1Selectively activates pituitary and hypothalamic GHSR-1a receptors via G-protein coupled phospholipase C.
2Elevates intracellular inositol trisphosphate (IP3) and calcium mobilization in somatotroph cells.
3Displays minimal binding affinity for corticotropin or prolactin receptors, ensuring endocrine selectivity.
4Demonstrates high synergy with GHRH analogues across pre-clinical endocrine assays.

Pre-Clinical Laboratory Handling

Laboratory Evaluation Guidelines

Considered one of the cleanest research tools for investigating somatotrophic secretory pulses and lipid metabolism without the confounding endocrine perturbations seen with GHRP-6 or GHRP-2.

Aseptic Dissolution & StorageUK Lab Standard

Recommended Diluent:Bacteriostatic 0.9% Benzyl Alcohol USP

Lyophilized Storage:-20°C desiccated (stable up to 24 months)

Reconstituted Storage:2°C to 8°C refrigerated; protect from light (stable 28 days)

Solution Stability:Enzymatically stable pentapeptide; keep tightly sealed in glass vials

Academic Biochemical Monograph

Receptor Affinity, SPPS Synthesis & Pharmacokinetics

Standardized chemical parameters, solid-phase synthesis criteria, and quantitative receptor affinity benchmarks for Ipamorelin.

Target ReceptorsBinding Kinetics

Primary Receptor Cascade

Ghrelin / Growth Hormone Secretagogue Receptor 1a (GHSR-1a)

Binding Affinity (Kd / Ki)

EC50 ≈ 1.3 nM (GHSR-1a selective agonist)

Receptor Family

Class A Rhodopsin-like G-Protein Coupled Receptor (GPCR)

Downstream Effectors

Gq/11 protein coupling and phospholipase C (PLC) activation
IP3-mediated intracellular Ca2+ mobilization
Selective pituitary GH exocytosis

SPPS MethodologyISO Criteria

Synthesis Protocol

Fmoc Solid-Phase Peptide Synthesis (SPPS)

N-Terminal / C-Terminal Mod

Free amine (AIB-) · Amidated (-NH2)

Acid Cleavage Cocktail

TFA / TIS / H2O (95:2.5:2.5 vol/vol)

HPLC Retention Standard

RP-C18: 12.1 min (0.1% TFA in H2O/ACN)

PharmacokineticsIn Vitro / In Vivo

Elimination Half-Life

t1/2 ≈ 2.0 hours

Primary Degradation Pathway

Hepatic microsomal metabolism and enzymatic peptidase cleavage

Bioavailability Profile

AIB and D-Phe modifications confer enzymatic stability

Renal Filtration Threshold

Rapid filtration (MW: 711.85 Da)

Quantitative Pre-Clinical & Clinical Evidence Matrix

Documented Biochemical Endpoints & Statistical Significance

Study Design	Cohort (n)	Measured Biological Endpoint	Statistical Significance	NCBI Citation
In Vitro Assay	n = 24 isolated rat pituitary cell models	Selective GH exocytosis vs ACTH and prolactin levels	p < 0.001 (absolute endocrine selectivity)	PMID 9849822

Peer-Reviewed Academic Citations

Indexed Scientific Literature

Ipamorelin, the first selective growth hormone secretagogue

European Journal of Endocrinology (1998) · Raun K, Hansen BS, Johansen PB, et al.

Key Finding: Characterized complete selectivity for GH release with zero effect on ACTH, cortisol, or prolactin levels.

PubMed PMID: 9849822

BIOCHEMICAL & REGULATORY Q&A

Curated Scientific Q&A for Ipamorelin

Authoritative pre-clinical, analytical, and statutory handling guidelines for laboratory researchers.

5 Curated Questions

Ipamorelin (Aib-His-D-2-Nal-D-Phe-Lys-NH2 Pentapeptide) is indexed in the CAS Registry under #170851-70-4. Its empirical molecular formula is C38H49N9O5 with a monoisotopic molecular weight of 711.86 g/mol. It is cataloged under the Growth Hormone Secretagogues & GHRH research classification.

Citation Benchmark: PubChem CID 9831659; British Pharmacopoeia Appendix III

Biological Pathway Correlates

More Reagents in Growth Hormone Secretagogues & GHRH

View Pathway Hub

Mixed Pre-ClinicalCAS 863288-34-0

CJC-1295

Modified Growth Hormone-Releasing Hormone (Mod GRF 1-29 / CJC-1295 No DAC)

C152H252N44O423367.97 g/molGrowth Hormone Secretagogues & GHRH

CJC-1295 (Mod GRF 1-29) is a synthetic 29-amino-acid analogue of human growth hormone-releasing factor featuring four D-amino acid substitutions to enhance metabolic stability against dipeptidyl peptidase-4 (DPP-4). Evaluated in UK endocrine research for pulsatile somatotroph signaling.

Verified UK Sourcing Partners:

PharmaGrade Store (≥99.2% (HPLC))Direct Peptides (≥99.0% (HPLC))Direct Sarms (≥98.6% (HPLC))Peptide Works (≥98.8% (HPLC))PharmaLab Global (≥99.4% (HPLC))

View Full Technical Monograph