What primary receptor cascades and in vitro signaling axes are modulated by Selank?

Pre-clinical studies indicate Selank engages primary pathways: Acts as a positive allosteric modulator at GABAA receptor complexes, enhancing inhibitory neurotransmission. Inhibits enkephalin-degrading enzymes in blood plasma and neural tissue, elevating endogenous opioid signaling. Additional investigations explore cellular migration, receptor binding affinities, and kinase activation in vitro.

What is the recommended reconstitution diluent and sterile handling protocol for Selank?

Lyophilized Selank cakes must be reconstituted using Sterile Deionized Water USP or Bacteriostatic Water. Introduce the diluent gently along the internal glass wall under a laminar flow hood, allowing passive dissolution without mechanical vortex shear or high-velocity liquid agitation.

Is Selank lawful to purchase and possess for laboratory research in the United Kingdom?

Yes. Non-scheduled synthetic research peptides like Selank are entirely legal to acquire, import, and hold for bona fide laboratory experimentation in the UK. However, under Regulation 46 of the Human Medicines Regulations 2012 (SI 2012/1916), supply or marketing for human clinical administration without MHRA authorization is strictly unlawful.

UK Academic MonographNeuropeptides & CNS SignalingIn Vitro Laboratory Only

Selank

Q: What is the CAS registry number, molecular formula, and mass of Selank?

Selank (Synthetic Tuftsin Heptapeptide Analogue (Thr-Lys-Pro-Arg-Pro-Gly-Pro)) is indexed in the CAS Registry under #129954-34-3. Its empirical molecular formula is C33H57N11O9 with a monoisotopic molecular weight of 751.88 g/mol. It is cataloged under the Neuropeptides & CNS Signaling research classification.

Q: What are the storage temperature thresholds and solution kinetics for Selank?

Lyophilized vials require desiccated storage at -20°C dark vacuum desiccator (stable 24 months). Once reconstituted into an aqueous solvent, the peptide solution must be stored at 2°C to 8°C refrigerated; protect from light (stable 30 days) protected from UV degradation. The laboratory stability window is Enzymatically stable heptapeptide; keep shielded from UV radiation. Avoid repeated freeze-thaw cycles.

Synthetic Tuftsin Heptapeptide Analogue (Thr-Lys-Pro-Arg-Pro-Gly-Pro)

PubChem Molecular SpecsValidated

CAS Registry:129954-34-3

Chemical Formula:C33H57N11O9

Monoisotopic MW:751.88 g/mol

PubChem CID:11765600

Primary Amino Acid Sequence (7 residues)

N-terminus → C-terminus

1Thr

2Lys

3Pro

4Arg

5Pro

6Gly

7Pro

Pre-Clinical Overview

Selank is a synthetic regulatory heptapeptide derived from the immunomodulatory tetrapeptide tuftsin, extended with a C-terminal Pro-Gly-Pro tripeptide. Examined in neuroimmunology laboratories for its allosteric modulation of GABAA receptors, enkephalinase inhibition, and IL-6 expression.

Independent Laboratory Audit

Verified UK Suppliers for Selank

5 Audited British & European Sources

Supplier	Assay Purity	Reagent Format	Dispatch Location	Notes
PharmaGrade Store	≥99.2% (HPLC)	spray	United Kingdom (Royal Mail 24)	High-purity lyophilized 5mg vials and calibrated intranasal atomizers.
Direct Peptides	≥99.0% (HPLC)	vial	UK Hub & Europe	Research grade lyophilized peptide vials with automated mass spectrometry confirmation.
Direct Sarms	≥98.6% (HPLC)	spray	United Kingdom Express	Analytical reference standard vials tested for neurochemical assays.
Peptide Works	≥98.9% (HPLC)	vial	Manchester UK Hub	Northern UK laboratory dispatched lyophilized reagent vials with sequence COA.
PharmaLab Global	≥99.3% (HPLC)	spray	London UK Hub	Triple-stage assayed vials and metered laboratory nasal spray units.

Biochemical Mechanisms of Action

Molecular Signaling Pathways

1Acts as a positive allosteric modulator at GABAA receptor complexes, enhancing inhibitory neurotransmission.
2Inhibits enkephalin-degrading enzymes in blood plasma and neural tissue, elevating endogenous opioid signaling.
3Regulates expression of pro-inflammatory cytokines IL-6 and TNF-alpha in mononuclear blood cells.
4Modulates brain serotonin and dopamine turnover rates without benzodiazepine-like sedation or tolerance.

Pre-Clinical Laboratory Handling

Laboratory Evaluation Guidelines

Stabilized against circulating peptidases by the Pro-Gly-Pro sequence. Frequently cataloged in neurotrophic assays alongside Semax to evaluate comparative cognitive and anxiolytic receptor pathways.

Aseptic Dissolution & StorageUK Lab Standard

Recommended Diluent:Sterile Deionized Water USP or Bacteriostatic Water

Lyophilized Storage:-20°C dark vacuum desiccator (stable 24 months)

Reconstituted Storage:2°C to 8°C refrigerated; protect from light (stable 30 days)

Solution Stability:Enzymatically stable heptapeptide; keep shielded from UV radiation

Academic Biochemical Monograph

Receptor Affinity, SPPS Synthesis & Pharmacokinetics

Standardized chemical parameters, solid-phase synthesis criteria, and quantitative receptor affinity benchmarks for Selank.

Target ReceptorsBinding Kinetics

Primary Receptor Cascade

GABAA Receptor Allosteric Modulation & Enkephalin Degradation Inhibition

Binding Affinity (Kd / Ki)

Allosteric modulation of GABAA chloride channels (EC50 ≈ 5.0 nM)

Receptor Family

Ligand-Gated Ion Channel Modulator & Peptidase Inhibitor

Downstream Effectors

Inhibition of carboxypeptidase N and neutral endopeptidase
Enkephalin preservation in cerebral cortex tissues
Interleukin-6 mRNA downregulation in immune cells

SPPS MethodologyISO Criteria

Synthesis Protocol

Fmoc Solid-Phase Peptide Synthesis (SPPS)

N-Terminal / C-Terminal Mod

Free amine (H-Thr-) · Free carboxylic acid (-Pro-OH)

Acid Cleavage Cocktail

TFA / TIS / H2O (95:2.5:2.5 vol/vol)

HPLC Retention Standard

RP-C18: 8.7 min (0.1% TFA in H2O/ACN)

PharmacokineticsIn Vitro / In Vivo

Elimination Half-Life

t1/2 ≈ 24 hours (stabilized by C-terminal Pro-Gly-Pro motif)

Primary Degradation Pathway

Tuftsin analog core protected by tripeptide tail

Bioavailability Profile

Neurochemistry assay benchmark

Renal Filtration Threshold

Rapid excretion (MW: 751.90 Da)

Quantitative Pre-Clinical & Clinical Evidence Matrix

Documented Biochemical Endpoints & Statistical Significance

Study Design	Cohort (n)	Measured Biological Endpoint	Statistical Significance	NCBI Citation
In Vitro Assay	n = 20 spleen cell cultures	Expression of IL-6 and immunoregulatory cytokines	p < 0.01 vs control	PMID 18577368
Preclinical In Vivo	n = 45 neurobehavioral rodent paradigms	Elevated plus-maze exploration and anxiolytic markers	p < 0.001	PMID 19803361

Peer-Reviewed Academic Citations

Indexed Scientific Literature

Selank administration affects the expression of some genes involved in GABAergic neurotransmission

Frontiers in Pharmacology (2016) · Volkova A, Mezentseva O, Tarasova L, et al.

Key Finding: Identified modulation of 64 genes participating in GABAergic receptor signaling and synaptic plasticity.

PubMed PMID: 27242531

BIOCHEMICAL & REGULATORY Q&A

Curated Scientific Q&A for Selank

Authoritative pre-clinical, analytical, and statutory handling guidelines for laboratory researchers.

5 Curated Questions

Selank (Synthetic Tuftsin Heptapeptide Analogue (Thr-Lys-Pro-Arg-Pro-Gly-Pro)) is indexed in the CAS Registry under #129954-34-3. Its empirical molecular formula is C33H57N11O9 with a monoisotopic molecular weight of 751.88 g/mol. It is cataloged under the Neuropeptides & CNS Signaling research classification.

Citation Benchmark: PubChem CID 11765600; British Pharmacopoeia Appendix III

Biological Pathway Correlates

More Reagents in Neuropeptides & CNS Signaling

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Human Trial DataCAS 80714-61-0

Semax

Heptapeptide ACTH(4-10) Analog (Met-Glu-His-Phe-Pro-Gly-Pro)

C37H51N9O10S813.92 g/molNeuropeptides & CNS Signaling

Semax is a synthetic regulatory heptapeptide structurally derived from an adrenocorticotropic hormone fragment (ACTH 4-10) stabilized with a C-terminal Pro-Gly-Pro tripeptide. Examined in neuropharmacology laboratories for its modulation of BDNF, trkB receptors, and cerebral monoamines.

Verified UK Sourcing Partners:

PharmaGrade Store (≥99.1% (HPLC))Direct Peptides (≥99.0% (HPLC))Direct Sarms (≥98.6% (HPLC))Peptide Works (≥98.9% (HPLC))PharmaLab Global (≥99.3% (HPLC))

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